RESEARCH PAPER
Correlation of copper/zinc ratio with superoxide dismutase activity and neutrophil-to-lymphocyte ratio in human immunodeficiency virus type 1 (HIV-1) infected subjects
Submission date: 2017-05-23
Final revision date: 2017-10-07
Acceptance date: 2017-10-08
Publication date: 2017-12-22
HIV & AIDS Review 2018;17(1):18-23
KEYWORDS
TOPICS
ABSTRACT
Introduction:
The important role of nutrition in the pathogenesis of human immunodeficiency virus type-1 (HIV-1) infection has not been sufficiently addressed in sub-Sahara Africa, where the prevalence of HIV infection is high. This study correlates copper-to-zinc ratio with activities of superoxide dismutase and calculated neutrophil-to-lymphocyte ratio in HIV-1-infected subjects.
Material and methods:
In the study, there were 120 confirmed HIV-1 positive subjects and 100 HIV-1 negative ambulatory healthy controls. Complete blood count, clusters of differentiation (CD4+) lymphocyte count, serum superoxide dismutase (SOD), catalase, copper, and zinc were evaluated using hematology analyzer, FACS flow cytometer, enzyme-linked immunosorbent assay, and atomic absorption spectrophotometer, respectively. Correlation between the measured variables was completed using Pearson correlation coefficient.
Results:
Serum copper (p < 0.001), catalase (p < 0.05), and neutrophil-to-lymphocyte ratio (NLR) (p < 0.001) were significantly higher, while serum zinc (p < 0.001) was lower in HIV-1 positive than control subjects. Copper/zinc ratio correlated with SOD (r = –0.228; p < 0.02), CD4+ (–0.235; p < 0.02), and NLR (r = 0.198; p < 0.05) in HIV infected subjects.
Conclusions:
Copper/zinc ratio correlated with NLR, SOD, and CD4+ cell count. Because of the adverse effects associated with imbalance of copper and zinc levels, nutritional status, antioxidant defense mechanism, and inflammatory markers may be assessed periodically in HIV-1-infected patients.
The important role of nutrition in the pathogenesis of human immunodeficiency virus type-1 (HIV-1) infection has not been sufficiently addressed in sub-Sahara Africa, where the prevalence of HIV infection is high. This study correlates copper-to-zinc ratio with activities of superoxide dismutase and calculated neutrophil-to-lymphocyte ratio in HIV-1-infected subjects.
Material and methods:
In the study, there were 120 confirmed HIV-1 positive subjects and 100 HIV-1 negative ambulatory healthy controls. Complete blood count, clusters of differentiation (CD4+) lymphocyte count, serum superoxide dismutase (SOD), catalase, copper, and zinc were evaluated using hematology analyzer, FACS flow cytometer, enzyme-linked immunosorbent assay, and atomic absorption spectrophotometer, respectively. Correlation between the measured variables was completed using Pearson correlation coefficient.
Results:
Serum copper (p < 0.001), catalase (p < 0.05), and neutrophil-to-lymphocyte ratio (NLR) (p < 0.001) were significantly higher, while serum zinc (p < 0.001) was lower in HIV-1 positive than control subjects. Copper/zinc ratio correlated with SOD (r = –0.228; p < 0.02), CD4+ (–0.235; p < 0.02), and NLR (r = 0.198; p < 0.05) in HIV infected subjects.
Conclusions:
Copper/zinc ratio correlated with NLR, SOD, and CD4+ cell count. Because of the adverse effects associated with imbalance of copper and zinc levels, nutritional status, antioxidant defense mechanism, and inflammatory markers may be assessed periodically in HIV-1-infected patients.
REFERENCES (58)
1.
Allard JP, Aghdassi E, Chau J, et al. Oxidative stress and plasma antioxidant micronutrients in humans. Am J Clin Nutr 1998; 67: 143-147.
2.
Abrams B, Duncan D, Hertz-Picciotto I. A prospective study of dietary intake and acquired immune deficiency syndrome in HIV seropositive homosexual men. J Acquir Immune Defic Syndr 1993; 6: 949-958.
3.
Malviya A, Hassan H, Hussain A. Correlation of CD4+T cell count with serum zinc, copper, and selenium in HIV positive individuals. Internet J Epidemiol 2008; 6: 1-6.
4.
Kassu A, Yabutani T, Mahmud ZH, et al. Alterations in serum levels of trace elements in tuberculosis HIV infection. Eur J Clin Nutr 2006; 60: 580-586.
5.
Roy SK, Raqib R, Khatun W, et al. Zinc supplementation in the management of shigellosis in malnourished children in Bangladesh. Eur J Clin Nutr 2004; 62: 849-855.
6.
Amare B, Tafess K, Moges F, et al. Levels of Serum Zinc, Copper and Copper/Zinc Ratio in Patients with Diarrhea and HIV Infection in Ethiopia. Vitam Trace Elem 2011; 1: 101.
7.
Emokpae MA, Mrakpor BA. Do Sex Differences in Respiratory Burst Enzyme Activities Exist in Human Immunodeficiency Virus-1 Infection? Med Scis 2016; 4: 1-8.
8.
Akinjinmi AA, Akingbade OA, Okerentugba PO, et al. Evaluation of selenium and zinc levels in seropositive HIV individuals in a tertiary Hospital in Osogbo, Osun State Nigeria. Academ Arena 2013; 5: 17-21.
9.
Elatif MA, Hassan EE, Bakhit SM, et al. Assessment of plasma zinc and copper levels among Sudanese HIV patients in Khartoum state. Int J Adv Pharm Biol Chem 2014; 3: 395-399.
10.
Drain PK, Kupka R, Mugusi F, et al. Micronutrients in HIV positive persons receiving highly active antiretroviral therapy. Am J Clin Nutr 2007; 85: 333-345.
11.
Ibeh IN, Nwokenna US. Studies on cations as biomarkers of mammalian immunity: prospects in disease control. J Natl Acad Adv Sci 2003; 2: 89-109.
12.
Kupka R, Msamanga GI, Spiegelman D, et al. Selenium status is associated with accelerated HIV disease progression among HIV-1-infected pregnant women in Tanzania. J Nutr 2004; 134: 2556-2560.
13.
Mao S, Huang S. Zinc and copper levels in bladder cancer: a systemic review and meta-analysis. Biol Trace Elem Res 2013; 153: 5-10.
14.
Poo JL, Romero RR, Robles JA, et al. Diagnostic value of copper/zinc ratio in digestive cancer: a case control study. Arch Med Res 1997; 28: 259-263.
15.
Ibeh IN, Nwokenna US, Onemu S, et al. Studies on disease markers in a Nigerian community. Natl J Sci 2002; 1: 20-25.
16.
Malapati BR, Nadeem DR, Patel B, et al. Serum zinc and copper levels in HIV positive patients. Eur J Parmaceut Med Res 2015; 2: 265-270.
17.
Osredkar J, Sustar N. Copper and zinc, biological role and significance of copper/zinc imbalance. J Clin Toxicol 2011; 5: 3.
18.
Minardi D, Scartozzi M, Montesi L, et al. Neutrophil-to-lymphocyte ratio may be associated with the outcome in patients with prostate cancer. Springer Plus 2015; 4: 255.
19.
Emokpae MA, Aruomaren A, Osime E. Relationship between Neutrophil-to-Lymphocyte Ratio and Inflammatory Markers in Sickle Cell Anaemia Patients with Proteinuria. Med Sci 2016; 4: 1-8.
20.
Pinato DJ, Stavraka C, Flynn MJ, et al. An inflammation-based score can optimize the selection of patients with advanced cancer considered for early phase clinical trial. PLos One 2014; 1-10: e83279.
21.
Li MX, Liu XM, Zhang XF, et al. Prognostic role of neutrophil-to-lymphocyte ratio in colorectal cancer: a systematic review. Int J Cancer 2014; 134: 2403-2413.
22.
Grivennikov SI, Greten FR, Karin M. Immunity, inflammation and cancer. Cell 2010; 140: 833-899.
23.
Zahorec R. Ratio of neutrophil to lymphocyte count – rapid and simple parameter of systemic inflammation and stress in critically ill. Bratisl Lek Listy 2001; 102: 5-14.
24.
Uadia PO, Emokpae MA. Implications of Oxidative Stress on Male Infertility. Transaction of the Nigerian Society of Biochemistry and Molecular Biology 2015; 1: 19-29.
25.
Failla ML. Trace elements and host defense: recent advances and continuing challenges. J Nutr 2003; 133: 1443S-1447S.
26.
Stern BR, Solioz M, Krewski D, et al. Copper and Human Health: biochemistry, genetics, and strategies for modeling dose-response relationships. J Toxicol Environ Health B Crit Rev 2007; 10: 157-222.
27.
Tomkins A. Assessing micronutrient status in the presence of inflammation. J Nutr 2003; 133: 1649S-1655S.
28.
Arinola OG, Adedapo KS, Kehinde AO, et al. Acute phase proteins, trace elements in asymptomatic human immunodeficiency virus infection in Nigerians. Afr J Med Med Sci 2004; 33: 317-322.
29.
Semba RD, Caiaffa WT, Graham NM, et al. Vitamin A deficiency and wasting as predictors of mortality in human immunodeficiency virus-infected injection drug users. J Infect Dis 1995; 171: 1196-1202.
30.
Coodley GO, Coodley MK, Nelson HD, et al. Micronutrient concentrations in the HIV wasting syndrome. AIDS 1993; 7: 1595-1600.
31.
Kupka R, Msamanga GI, Donna S, et al. Selenium status is associated with accelerated HIV disease progression among HIV-1-infected pregnant women in Tanzania. J Nutr 2004; 134: 2556-2560.
32.
Graham NM, Sorensen D, Odaka N, et al. Relationship of serum copper and zinc levels to HIV-1 seropostivity and progression to AIDS. J Acquir Immuno Defic Syndr 1991; 4: 976-980.
33.
Khalili A, Soudbakhsh A, Hajiabdolbaghi M, et al. Nutritional status and serum zinc and selenium levels in Iranian HIV infected individuals. BMC Infect Dis 2008; 8: 165.
34.
Moreno T, Artacho R, Navarro M, et al. Serum copper concentration in HIV-infection patients and relationships with other biochemical indices. Sci Total Environ 1998; 217: 21-26.
35.
Ucar SK, Coker M, Sözmen E, et al. An association among iron, copper, zinc, and selenium, and antioxidative status in dyslipidemic pediatric patients with glycogen storage disease types IA and III. J Trace Elem Med Biol 2010; 24: 42-45.
36.
Ferns GA, Lamb DJ, Taylor A. The possible role of copper ions in atherogenesis: the Blue Janus. Atherosclerosis 1997; 133: 139-152.
37.
Mazur A, Gueux E, Bureau I, et al. Copper deficiency and lipoprotein oxidation. Atherosclerosis 1998; 137: 443-445.
38.
Ferencík M, Ebringer L. Modulatory effects of selenium and zinc on the immune system. Folia Microbiol (Praha) 2003; 48: 417-426.
39.
Fraker PJ, King LE, Laakko T, et al. The dynamic link between the integrity of the immune system and zinc status. J Nutr 2000; 130: 1399S-1406S.
41.
Fawzi W. Micronutrients and human immunodeficiency virus type 1 disease progression among adults and children. Clin Infect Dis 2003; 37: S112-S116.
42.
Abdulla M. Trace elements and free radicals in health and disease: Essential and Toxic Trace Elements in Human Health and Disease. Alan R. Liss, New York 1998; p. 277-292.
43.
Chandra RK. Effect of vitamin and trace-element supplementation on immune responses and infection in elderly subjects. Lancet 1992; 340: 1124-1127.
44.
Beisel WR. Metabolic responses of the host to infections: Textbook of pediatric infectious diseases. WB Saunders Co, Philadelphia 1998; p. 34-40.
45.
Olivares M, Uauy R. Copper as an essential nutrient. Am J Clin Nutr 1996; 63: 791S-96S.
46.
Bogden JD, Kemp FW, Han S, et al. Status of selected nutrients and progression of human immunodeficiency virus type 1 infection. Am J Clin Nutr 2000; 72: 809-815.
47.
Bogden JD, Baker H, Frank O, et al. Micronutrient status and immunodeficiency virus (HIV) infection, micronutrients and immune function: cytokines and metabolism. Ann NY Acad Sci 1990; 58: 189-195.
48.
Lai H, Lai S, Shor-Posner G, et al. Plasma zinc, copper, and mortality in HIV-1 infected homosexual men. Int Conf AIDS 1998; 12: 34-35.
49.
Quiros-Rolden E, Raffetti E, Donato F, et al. Neutrophil to lymphocyte ratio and cardiovascular disease incidence in HIV-infected patients: a population-based cohort study. PLos One 2016; 11: 1-11.
50.
Raffetti E, Donato F, Pezzoli C, et al. Systemic Inflammation-Based Biomarkers and Survival in HIV-Positive Subjects With Solid Cancer in an Italian Multicener Study. J Acquir Immune Defic Syndr 2015; 69: 585-592.
51.
Raffetti E, Donato F, Castelnuovo F, et al. The prognostic role of systemic inflammatory markers on HIV-infected patients with non-Hodgkin lymphoma, a multicenter cohort study. J Transl Med 2015; 13: 89.
52.
Tenorio AR, Zheng Y, Bosch RJ, et al. Soluble markers of inflammation and coagulation but not T-cell activation predict non-AIDS-defining morbid events during suppressive antiretroviral treatment. J Infect Dis 2014; 210: 1248-1259.
53.
Ross AC, Rizk N, O’Riordan MA, et al. Relationship between inflammatory markers, endothelial activation markers, and carotid intima-media thickness in HIV-infected patients receiving antiretroviral therapy. Clin Infect Dis 2009; 49: 1119-1127.
54.
Benites-Zapata VA, Hernandez AV, Nagarajan V, et al. Usefulness of neutrophil-to-lymphocyte ratio in risk stratification of patients with advanced heart failure. Am J Cardiol 2015; 115: 57-61.
55.
Park JJ, Jang HJ, Oh IY, et al. Prognostic value of neutrophil to lymphocyte ratio in patients presenting with ST-elevation myocardial infarction undergoing primary percutaneous coronary intervention. Am J Cardiol 2013; 111: 636-642.
56.
Pugliese C, Patin RV, Palchetti CZ, et al. Assessment of antioxidants status and superoxide dismutase activity in HIV-infected children. Braz J Infect Dis 2014; 18: 481-486.
57.
Dworkin BM. Selenium deficiency in HIV infection and the acquired immunodeficiency syndrome (AIDS). Chem Biol Interact 1994; 91: 181-186.
58.
Seifried HE, Anderson DE, Sorkin BC, et al. Free radicals: the pros and cons of antioxidants. American Society for Nutritional Sciences. J Nutr 2004; 134: 3143-3163.
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