Aim: To determine the incidence of HIV among the adult population of selected government areas in Osun State Nigeria. Background: Osun State is one of the states in Nigeria with the lowest HIV rates in Nigeria. Figures for 2013 revealed 2.7%. It is important that this figure is monitored constantly to ensure it does not rise above this and to enable health care organizations make necessary and timely interventions if and when necessary. Also surveys such as this provide vital data for international world health organizations in order that monitoring is made easier when they have access to data produced by such surveys. Materials and methods: Trained counseling teams visited the 15 selected local government areas and with the cooperation of the recognized community leaders the HIV status was determined among adults living in these areas. Three testing kits from different manufacturers were utilized. Unigold, Statpak and Determine using recognized standard methods. The results would be subjected to statistical analysis SPSS version 10 was used for the statistical analysis. Results and conclusion: Total number of subjects screened was 3250, of which 1144 (35.2%) were male and 2106 (64.8%) were female. The males were aged 36.72 _ 21.19 and females 39.30 _ 19.35. The screening kits used were Unigold, Determine and Statpak. Those positive were 18 (0.6%) with the female sex predominating. More intensive efforts should be made in terms of prevention to maintain and if possible reduce these figures.